Dissociation Constant in presence of Noncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary
Formula Used
Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1))
Ki = (I/((Vmax/Vmaxapp)-1))
This formula uses 4 Variables
Variables Used
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Apparent Maximum Rate - (Measured in Mole per Cubic Meter Second) - Apparent Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration in presence of non-competitive inhibitor.
STEP 1: Convert Input(s) to Base Unit
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Apparent Maximum Rate: 21 Mole per Liter Second --> 21000 Mole per Cubic Meter Second (Check conversion here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
Ki = (I/((Vmax/Vmaxapp)-1)) --> (9000/((40000/21000)-1))
Evaluating ... ...
Ki = 9947.36842105263
STEP 3: Convert Result to Output's Unit
9947.36842105263 Mole per Cubic Meter -->9.94736842105263 Mole per Liter (Check conversion here)
FINAL ANSWER
9.94736842105263 9.947368 Mole per Liter <-- Enzyme Inhibitor Dissociation Constant
(Calculation completed in 00.004 seconds)

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Created by Prashant Singh
K J Somaiya College of science (K J Somaiya), Mumbai
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9 Noncompetitive Inhibitor Calculators

Dissociation Constant given Enzyme Substrate Complex Concentration
Go Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)
Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor
Go Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
Initial Enzyme Concentration in presence of Noncompetitive Inhibitor
Go Initial Enzyme Concentration = (Apparent Initial Enzyme Concentration*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
Dissociation Constant given Apparent Initial Enzyme Concentration
Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1))
Apparent Michaelis Menten constant given Inhibitor's Dissociation Constant
Go Apparent Michaelis Constant = Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))
Apparent Maximum Rate in presence of Noncompetitive Inhibitor
Go Apparent Maximum Rate = (Maximum Rate/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
Dissociation Constant in presence of Noncompetitive Inhibitor
Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1))
Maximum Rate in presence of Noncompetitive Inhibitor
Go Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
Inhibitor Concentration in presence of Noncompetitive Inhibitor
Go Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant

Dissociation Constant in presence of Noncompetitive Inhibitor Formula

Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1))
Ki = (I/((Vmax/Vmaxapp)-1))

What is Non-competitive inhibition?

Non-competitive inhibition is a type of enzyme inhibition where the inhibitor reduces the activity of the enzyme and binds equally well to the enzyme whether or not it has already bound the substrate. In the presence of a non-competitive inhibitor, the apparent enzyme affinity is equivalent to the actual affinity because the inhibitor binds to both the enzyme and the enzyme-substrate complex equally so that the equilibrium is maintained. However, since some enzyme is always inhibited from converting the substrate to product, the effective enzyme concentration is lowered.

How to Calculate Dissociation Constant in presence of Noncompetitive Inhibitor?

Dissociation Constant in presence of Noncompetitive Inhibitor calculator uses Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1)) to calculate the Enzyme Inhibitor Dissociation Constant, The Dissociation constant in presence of noncompetitive inhibitor formula is defined as a relation with the apparent maximum rate of the system, inhibitor concentration, and maximum rate of the system. Enzyme Inhibitor Dissociation Constant is denoted by Ki symbol.

How to calculate Dissociation Constant in presence of Noncompetitive Inhibitor using this online calculator? To use this online calculator for Dissociation Constant in presence of Noncompetitive Inhibitor, enter Inhibitor Concentration (I), Maximum Rate (Vmax) & Apparent Maximum Rate (Vmaxapp) and hit the calculate button. Here is how the Dissociation Constant in presence of Noncompetitive Inhibitor calculation can be explained with given input values -> 0.009947 = (9000/((40000/21000)-1)).

FAQ

What is Dissociation Constant in presence of Noncompetitive Inhibitor?
The Dissociation constant in presence of noncompetitive inhibitor formula is defined as a relation with the apparent maximum rate of the system, inhibitor concentration, and maximum rate of the system and is represented as Ki = (I/((Vmax/Vmaxapp)-1)) or Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1)). The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system, The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration & Apparent Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration in presence of non-competitive inhibitor.
How to calculate Dissociation Constant in presence of Noncompetitive Inhibitor?
The Dissociation constant in presence of noncompetitive inhibitor formula is defined as a relation with the apparent maximum rate of the system, inhibitor concentration, and maximum rate of the system is calculated using Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1)). To calculate Dissociation Constant in presence of Noncompetitive Inhibitor, you need Inhibitor Concentration (I), Maximum Rate (Vmax) & Apparent Maximum Rate (Vmaxapp). With our tool, you need to enter the respective value for Inhibitor Concentration, Maximum Rate & Apparent Maximum Rate and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Enzyme Inhibitor Dissociation Constant?
In this formula, Enzyme Inhibitor Dissociation Constant uses Inhibitor Concentration, Maximum Rate & Apparent Maximum Rate. We can use 2 other way(s) to calculate the same, which is/are as follows -
  • Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1))
  • Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)
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