Efficiency of Energy Transfer using Photobleaching Decay Time Constant Solution

STEP 0: Pre-Calculation Summary
Formula Used
Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)
E = 1-(ζpb/ζpbA)
This formula uses 3 Variables
Variables Used
Efficiency of Energy Transfer - The Efficiency of Energy Transfer describes the efficiency of energy transfer between two light-sensitive molecules (chromophores).
Photobleaching Decay Time Constant - The Photobleaching Decay Time Constant is the photobleaching decay time constant of the donor in the absence of the acceptor.
Photobleaching Decay Time Constant with FRET - The Photobleaching Decay Time Constant with FRET is the photobleaching decay time constant of the donor in the presence of the acceptor.
STEP 1: Convert Input(s) to Base Unit
Photobleaching Decay Time Constant: 0.002 --> No Conversion Required
Photobleaching Decay Time Constant with FRET: 0.003 --> No Conversion Required
STEP 2: Evaluate Formula
Substituting Input Values in Formula
E = 1-(ζpbpbA) --> 1-(0.002/0.003)
Evaluating ... ...
E = 0.333333333333333
STEP 3: Convert Result to Output's Unit
0.333333333333333 --> No Conversion Required
FINAL ANSWER
0.333333333333333 0.333333 <-- Efficiency of Energy Transfer
(Calculation completed in 00.004 seconds)

Credits

Created by Abhijit gharphalia
national institute of technology meghalaya (NIT Meghalaya), Shillong
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National University of Judicial Science (NUJS), Kolkata
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11 Förster resonance energy transfer Calculators

Forster Critical Distance
Go Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6)
Efficiency of Energy Transfer using Rate of Energy Transfer
Go Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)
Donor Lifetime with FRET using Rate of Energy and Transitions
Go Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)
Rate of Energy Transfer using Distances and Donor Lifetime
Go Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6
Efficiency of Energy Transfer using Photobleaching Decay Time Constant
Go Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)
Efficiency of Energy Transfer using Distances
Go Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)
Efficiency of Energy Transfer using Fluorescence Intensity of Donor
Go Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)
Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime
Go Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)
Donor Lifetime using Rates of Transitions
Go Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)
Fluorescence Quantum Yield in FRET
Go Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed
Efficiency of Energy Transfer using Donor Lifetime
Go Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)

Efficiency of Energy Transfer using Photobleaching Decay Time Constant Formula

Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)
E = 1-(ζpb/ζpbA)

What is photobleaching?

In optics, photobleaching is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce. This is caused by cleaving of covalent bonds or non-specific reactions between the fluorophore and surrounding molecules.

How to Calculate Efficiency of Energy Transfer using Photobleaching Decay Time Constant?

Efficiency of Energy Transfer using Photobleaching Decay Time Constant calculator uses Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET) to calculate the Efficiency of Energy Transfer, The Efficiency of Energy Transfer using Photobleaching Decay Time Constant formula is defined as the ratio of the difference of photobleaching decay time constant in presence and absence of acceptor respectively, to the photobleaching decay time constant in the presence of acceptor. Efficiency of Energy Transfer is denoted by E symbol.

How to calculate Efficiency of Energy Transfer using Photobleaching Decay Time Constant using this online calculator? To use this online calculator for Efficiency of Energy Transfer using Photobleaching Decay Time Constant, enter Photobleaching Decay Time Constant pb) & Photobleaching Decay Time Constant with FRET pbA) and hit the calculate button. Here is how the Efficiency of Energy Transfer using Photobleaching Decay Time Constant calculation can be explained with given input values -> 0.333333 = 1-(0.002/0.003).

FAQ

What is Efficiency of Energy Transfer using Photobleaching Decay Time Constant?
The Efficiency of Energy Transfer using Photobleaching Decay Time Constant formula is defined as the ratio of the difference of photobleaching decay time constant in presence and absence of acceptor respectively, to the photobleaching decay time constant in the presence of acceptor and is represented as E = 1-(ζpbpbA) or Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET). The Photobleaching Decay Time Constant is the photobleaching decay time constant of the donor in the absence of the acceptor & The Photobleaching Decay Time Constant with FRET is the photobleaching decay time constant of the donor in the presence of the acceptor.
How to calculate Efficiency of Energy Transfer using Photobleaching Decay Time Constant?
The Efficiency of Energy Transfer using Photobleaching Decay Time Constant formula is defined as the ratio of the difference of photobleaching decay time constant in presence and absence of acceptor respectively, to the photobleaching decay time constant in the presence of acceptor is calculated using Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET). To calculate Efficiency of Energy Transfer using Photobleaching Decay Time Constant, you need Photobleaching Decay Time Constant pb) & Photobleaching Decay Time Constant with FRET pbA). With our tool, you need to enter the respective value for Photobleaching Decay Time Constant & Photobleaching Decay Time Constant with FRET and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Efficiency of Energy Transfer?
In this formula, Efficiency of Energy Transfer uses Photobleaching Decay Time Constant & Photobleaching Decay Time Constant with FRET. We can use 5 other way(s) to calculate the same, which is/are as follows -
  • Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)
  • Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)
  • Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)
  • Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)
  • Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)
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