Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium Solution

STEP 0: Pre-Calculation Summary
Formula Used
Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration))
ES = (kf*[E0]*S)/(kr+(kf*S))
This formula uses 5 Variables
Variables Used
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Forward Rate Constant - (Measured in 1 Per Second) - The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Reverse Rate Constant - (Measured in Mole per Cubic Meter Second) - The Reverse Rate Constant is defined as the rate constant for the backward reaction.
STEP 1: Convert Input(s) to Base Unit
Forward Rate Constant: 6.9 1 Per Second --> 6.9 1 Per Second No Conversion Required
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Reverse Rate Constant: 20 Mole per Liter Second --> 20000 Mole per Cubic Meter Second (Check conversion here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
ES = (kf*[E0]*S)/(kr+(kf*S)) --> (6.9*100000*1500)/(20000+(6.9*1500))
Evaluating ... ...
ES = 34102.1416803954
STEP 3: Convert Result to Output's Unit
34102.1416803954 Mole per Cubic Meter -->34.1021416803954 Mole per Liter (Check conversion here)
FINAL ANSWER
34.1021416803954 34.10214 Mole per Liter <-- Enzyme Substrate Complex Concentration
(Calculation completed in 00.004 seconds)

Credits

Created by Prashant Singh
K J Somaiya College of science (K J Somaiya), Mumbai
Prashant Singh has created this Calculator and 700+ more calculators!
Verified by Prerana Bakli
University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA
Prerana Bakli has verified this Calculator and 1600+ more calculators!

21 Complex Concentration Calculators

Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium
Go Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration))
Initial Enzyme Concentration in Enzymatic Reaction Mechanism
Go Initial Enzyme Concentration = ((Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration))+Enzyme Substrate Complex Concentration
Substrate Concentration in Enzymatic Reaction Mechanism
Go Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants
Go Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)
Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants
Go Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration)
Initial Enzyme Concentration given Catalytic Rate Constant and Dissociation Rate Constants
Go Initial Enzyme Concentration = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)
Substrate Concentration given Catalytic Rate Constant and Dissociation Rate Constants
Go Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration
Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration given Dissociation Rate Constant
Go Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)
Initial Enzyme Concentration given Dissociation Rate Constant
Go Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)
Enzyme Substrate Complex Concentration given Dissociation Rate Constant
Go Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)
Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration
Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
Initial Enzyme Concentration at Low Substrate Concentration
Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)
Inhibitor Concentration given Apparent Initial Enzyme Concentration
Go Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant
Substrate Concentration given Maximum Rate and Dissociation Rate Constant
Go Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)
Substrate Concentration given Maximum Rate at Low Concentration
Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate
Inhibitor Concentration given Modifying Factor of Enzyme Substrate Complex
Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant
Inhibitor Concentration given Enzyme Substrate Modifying Factor
Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant
Inhibitor Concentration given Modifying Factor of Enzyme
Go Inhibitor Concentration = (Enzyme Modifying Factor-1)*Enzyme Inhibitor Dissociation Constant
Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate
Go Enzyme Inhibitor Complex Concentration = (Initial Reaction Rate/Final Rate Constant)
Initial Enzyme Concentration given Rate Constant and Maximum Rate
Go Initial Enzyme Concentration = Maximum Rate/Final Rate Constant

Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium Formula

Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration))
ES = (kf*[E0]*S)/(kr+(kf*S))

When a chemical system is at equilibrium?

In a chemical reaction, chemical equilibrium is the state in which both the reactants and products are present in concentrations which have no further tendency to change with time, so that there is no observable change in the properties of the system.

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium?

Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium calculator uses Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration)) to calculate the Enzyme Substrate Complex Concentration, The Enzyme substrate complex Concentration in instantaneous chemical equilibrium formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and substrate. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to calculate Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium using this online calculator? To use this online calculator for Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium, enter Forward Rate Constant (kf), Initial Enzyme Concentration ([E0]), Substrate Concentration (S) & Reverse Rate Constant (kr) and hit the calculate button. Here is how the Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium calculation can be explained with given input values -> 0.034102 = (6.9*100000*1500)/(20000+(6.9*1500)).

FAQ

What is Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium?
The Enzyme substrate complex Concentration in instantaneous chemical equilibrium formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and substrate and is represented as ES = (kf*[E0]*S)/(kr+(kf*S)) or Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration)). The Forward Rate Constant is defined as the rate constant for the forward occurring reaction, The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Substrate Concentration is the number of moles of substrate per liter solution & The Reverse Rate Constant is defined as the rate constant for the backward reaction.
How to calculate Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium?
The Enzyme substrate complex Concentration in instantaneous chemical equilibrium formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and substrate is calculated using Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration)). To calculate Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium, you need Forward Rate Constant (kf), Initial Enzyme Concentration ([E0]), Substrate Concentration (S) & Reverse Rate Constant (kr). With our tool, you need to enter the respective value for Forward Rate Constant, Initial Enzyme Concentration, Substrate Concentration & Reverse Rate Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Enzyme Substrate Complex Concentration?
In this formula, Enzyme Substrate Complex Concentration uses Forward Rate Constant, Initial Enzyme Concentration, Substrate Concentration & Reverse Rate Constant. We can use 1 other way(s) to calculate the same, which is/are as follows -
  • Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)
Let Others Know
Facebook
Twitter
Reddit
LinkedIn
Email
WhatsApp
Copied!