Inhibitor Concentration in presence of Noncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary
Formula Used
Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant
I = ((Vmax/Vmaxapp)-1)*Ki
This formula uses 4 Variables
Variables Used
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Apparent Maximum Rate - (Measured in Mole per Cubic Meter Second) - Apparent Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration in presence of non-competitive inhibitor.
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
STEP 1: Convert Input(s) to Base Unit
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Apparent Maximum Rate: 21 Mole per Liter Second --> 21000 Mole per Cubic Meter Second (Check conversion here)
Enzyme Inhibitor Dissociation Constant: 19 Mole per Liter --> 19000 Mole per Cubic Meter (Check conversion here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
I = ((Vmax/Vmaxapp)-1)*Ki --> ((40000/21000)-1)*19000
Evaluating ... ...
I = 17190.4761904762
STEP 3: Convert Result to Output's Unit
17190.4761904762 Mole per Cubic Meter -->17.1904761904762 Mole per Liter (Check conversion here)
FINAL ANSWER
17.1904761904762 17.19048 Mole per Liter <-- Inhibitor Concentration
(Calculation completed in 00.004 seconds)

Credits

Created by Prashant Singh
K J Somaiya College of science (K J Somaiya), Mumbai
Prashant Singh has created this Calculator and 700+ more calculators!
Verified by Prerana Bakli
University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA
Prerana Bakli has verified this Calculator and 1600+ more calculators!

9 Noncompetitive Inhibitor Calculators

Dissociation Constant given Enzyme Substrate Complex Concentration
Go Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)
Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor
Go Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
Initial Enzyme Concentration in presence of Noncompetitive Inhibitor
Go Initial Enzyme Concentration = (Apparent Initial Enzyme Concentration*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
Dissociation Constant given Apparent Initial Enzyme Concentration
Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1))
Apparent Michaelis Menten constant given Inhibitor's Dissociation Constant
Go Apparent Michaelis Constant = Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))
Apparent Maximum Rate in presence of Noncompetitive Inhibitor
Go Apparent Maximum Rate = (Maximum Rate/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
Dissociation Constant in presence of Noncompetitive Inhibitor
Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1))
Maximum Rate in presence of Noncompetitive Inhibitor
Go Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
Inhibitor Concentration in presence of Noncompetitive Inhibitor
Go Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant

Inhibitor Concentration in presence of Noncompetitive Inhibitor Formula

Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant
I = ((Vmax/Vmaxapp)-1)*Ki

What is Non-competitive inhibition?

Non-competitive inhibition is a type of enzyme inhibition where the inhibitor reduces the activity of the enzyme and binds equally well to the enzyme whether or not it has already bound the substrate. In the presence of a non-competitive inhibitor, the apparent enzyme affinity is equivalent to the actual affinity because the inhibitor binds to both the enzyme and the enzyme-substrate complex equally so that the equilibrium is maintained. However, since some enzyme is always inhibited from converting the substrate to product, the effective enzyme concentration is lowered.

How to Calculate Inhibitor Concentration in presence of Noncompetitive Inhibitor?

Inhibitor Concentration in presence of Noncompetitive Inhibitor calculator uses Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant to calculate the Inhibitor Concentration, The Inhibitor concentration in presence of noncompetitive inhibitor formula is defined as relation with the maximum rate of the system, apparent maximum rate, and with dissociation constant. Inhibitor Concentration is denoted by I symbol.

How to calculate Inhibitor Concentration in presence of Noncompetitive Inhibitor using this online calculator? To use this online calculator for Inhibitor Concentration in presence of Noncompetitive Inhibitor, enter Maximum Rate (Vmax), Apparent Maximum Rate (Vmaxapp) & Enzyme Inhibitor Dissociation Constant (Ki) and hit the calculate button. Here is how the Inhibitor Concentration in presence of Noncompetitive Inhibitor calculation can be explained with given input values -> 0.01719 = ((40000/21000)-1)*19000.

FAQ

What is Inhibitor Concentration in presence of Noncompetitive Inhibitor?
The Inhibitor concentration in presence of noncompetitive inhibitor formula is defined as relation with the maximum rate of the system, apparent maximum rate, and with dissociation constant and is represented as I = ((Vmax/Vmaxapp)-1)*Ki or Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant. The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration, Apparent Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration in presence of non-competitive inhibitor & The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
How to calculate Inhibitor Concentration in presence of Noncompetitive Inhibitor?
The Inhibitor concentration in presence of noncompetitive inhibitor formula is defined as relation with the maximum rate of the system, apparent maximum rate, and with dissociation constant is calculated using Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant. To calculate Inhibitor Concentration in presence of Noncompetitive Inhibitor, you need Maximum Rate (Vmax), Apparent Maximum Rate (Vmaxapp) & Enzyme Inhibitor Dissociation Constant (Ki). With our tool, you need to enter the respective value for Maximum Rate, Apparent Maximum Rate & Enzyme Inhibitor Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
Let Others Know
Facebook
Twitter
Reddit
LinkedIn
Email
WhatsApp
Copied!