Inhibitor Concentration in Presence of Uncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary
Formula Used
Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration
I = (Ki'*ESI)/ES
This formula uses 4 Variables
Variables Used
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Substrate Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.
Enzyme Substrate Inhibitor Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
STEP 1: Convert Input(s) to Base Unit
Enzyme Substrate Dissociation Constant: 15 Mole per Liter --> 15000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Inhibitor Complex Concentration: 2.5 Mole per Liter --> 2500 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
I = (Ki'*ESI)/ES --> (15000*2500)/10000
Evaluating ... ...
I = 3750
STEP 3: Convert Result to Output's Unit
3750 Mole per Cubic Meter -->3.75 Mole per Liter (Check conversion here)
FINAL ANSWER
3.75 Mole per Liter <-- Inhibitor Concentration
(Calculation completed in 00.004 seconds)

Credits

Created by Prashant Singh
K J Somaiya College of science (K J Somaiya), Mumbai
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University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA
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12 Uncompetitive Inhibitor Calculators

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor
Go Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration)
Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant
Go Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant)
Initial Reaction Rate in presence of Uncompetitive Inhibitor
Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))
Maximum Reaction Rate in Presence of Uncompetitive Inhibitor
Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration
Substrate Concentration in presence of Uncompetitive Inhibitor
Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))
Michaelis Constant in Presence of Uncompetitive Inhibitor
Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate
Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor
Go Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant
Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor
Go Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration
Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor
Go Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration
Inhibitor Concentration in Presence of Uncompetitive Inhibitor
Go Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration
Enzyme Substrate Modifying Factor given Enzyme Substrate Dissociation Constant
Go Enzyme Substrate Modifying Factor = (1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant))
Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor
Go Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)

Inhibitor Concentration in Presence of Uncompetitive Inhibitor Formula

Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration
I = (Ki'*ESI)/ES

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Inhibitor Concentration in Presence of Uncompetitive Inhibitor?

Inhibitor Concentration in Presence of Uncompetitive Inhibitor calculator uses Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration to calculate the Inhibitor Concentration, The Inhibitor concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different enzyme species. Inhibitor Concentration is denoted by I symbol.

How to calculate Inhibitor Concentration in Presence of Uncompetitive Inhibitor using this online calculator? To use this online calculator for Inhibitor Concentration in Presence of Uncompetitive Inhibitor, enter Enzyme Substrate Dissociation Constant (Ki'), Enzyme Substrate Inhibitor Complex Concentration (ESI) & Enzyme Substrate Complex Concentration (ES) and hit the calculate button. Here is how the Inhibitor Concentration in Presence of Uncompetitive Inhibitor calculation can be explained with given input values -> 0.00375 = (15000*2500)/10000.

FAQ

What is Inhibitor Concentration in Presence of Uncompetitive Inhibitor?
The Inhibitor concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different enzyme species and is represented as I = (Ki'*ESI)/ES or Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration. The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product, The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex & The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
How to calculate Inhibitor Concentration in Presence of Uncompetitive Inhibitor?
The Inhibitor concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different enzyme species is calculated using Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration. To calculate Inhibitor Concentration in Presence of Uncompetitive Inhibitor, you need Enzyme Substrate Dissociation Constant (Ki'), Enzyme Substrate Inhibitor Complex Concentration (ESI) & Enzyme Substrate Complex Concentration (ES). With our tool, you need to enter the respective value for Enzyme Substrate Dissociation Constant, Enzyme Substrate Inhibitor Complex Concentration & Enzyme Substrate Complex Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
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