Yield of Protein Solution

STEP 0: Pre-Calculation Summary
Formula Used
The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase)
Yyield of protein = (Vtop phase*O.Dtop phase)/(Vbottom phase*ODbottom phase)
This formula uses 5 Variables
Variables Used
The yield of protein - The yield of protein is the amount of protein extracted by aqueous two phase extraction.
The Volume of top phase - (Measured in Cubic Meter) - The Volume of top phase is determined by the initial ratio of the two phases and the conditions of the extraction process.
The optical density of top phase - (Measured in Cubic Meter) - The optical density of top phase is the optical density of top phase extracts in aqueous two phase extraction.
The Volume of bottom phase - (Measured in Cubic Meter) - The Volume of bottom phase is the volume of liquid collected in the bottom phase after two phase extraction.
The optical density of bottom phase - (Measured in Cubic Meter) - The optical density of bottom phase the optical denisty of the bottom phase extracts in aqeous two phase extraction.
STEP 1: Convert Input(s) to Base Unit
The Volume of top phase: 25 Milliliter --> 2.5E-05 Cubic Meter (Check conversion here)
The optical density of top phase: 25 Milliliter --> 2.5E-05 Cubic Meter (Check conversion here)
The Volume of bottom phase: 20 Milliliter --> 2E-05 Cubic Meter (Check conversion here)
The optical density of bottom phase: 20 Milliliter --> 2E-05 Cubic Meter (Check conversion here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
Yyield of protein = (Vtop phase*O.Dtop phase)/(Vbottom phase*ODbottom phase) --> (2.5E-05*2.5E-05)/(2E-05*2E-05)
Evaluating ... ...
Yyield of protein = 1.5625
STEP 3: Convert Result to Output's Unit
1.5625 --> No Conversion Required
FINAL ANSWER
1.5625 <-- The yield of protein
(Calculation completed in 00.004 seconds)

Credits

Created by harykrishnan
SRM Institute of Science and Technology (SRMIST), Chennai
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National University of Judicial Science (NUJS), Kolkata
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Yield of Protein Formula

The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase)
Yyield of protein = (Vtop phase*O.Dtop phase)/(Vbottom phase*ODbottom phase)

What is Aqueous Two Phase Extraction ?

Aqueous two-phase extraction is a liquid-liquid separation technique that uses two immiscible aqueous solutions to partition and separate biomolecules or other substances based on their affinity for the different phases.

What is Protein Yield ?

Protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases.

How to Calculate Yield of Protein?

Yield of Protein calculator uses The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase) to calculate the The yield of protein, The Yield of Protein is the amount of protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases. The yield of protein is denoted by Yyield of protein symbol.

How to calculate Yield of Protein using this online calculator? To use this online calculator for Yield of Protein, enter The Volume of top phase (Vtop phase), The optical density of top phase (O.Dtop phase), The Volume of bottom phase (Vbottom phase) & The optical density of bottom phase (ODbottom phase) and hit the calculate button. Here is how the Yield of Protein calculation can be explained with given input values -> 1.25 = (2.5E-05*2.5E-05)/(2E-05*2E-05).

FAQ

What is Yield of Protein?
The Yield of Protein is the amount of protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases and is represented as Yyield of protein = (Vtop phase*O.Dtop phase)/(Vbottom phase*ODbottom phase) or The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase). The Volume of top phase is determined by the initial ratio of the two phases and the conditions of the extraction process, The optical density of top phase is the optical density of top phase extracts in aqueous two phase extraction, The Volume of bottom phase is the volume of liquid collected in the bottom phase after two phase extraction & The optical density of bottom phase the optical denisty of the bottom phase extracts in aqeous two phase extraction.
How to calculate Yield of Protein?
The Yield of Protein is the amount of protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases is calculated using The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase). To calculate Yield of Protein, you need The Volume of top phase (Vtop phase), The optical density of top phase (O.Dtop phase), The Volume of bottom phase (Vbottom phase) & The optical density of bottom phase (ODbottom phase). With our tool, you need to enter the respective value for The Volume of top phase, The optical density of top phase, The Volume of bottom phase & The optical density of bottom phase and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
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