## Yield of Protein Solution

STEP 0: Pre-Calculation Summary
Formula Used
The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase)
Yyield of protein = (Vtop phase*O.Dtop phase)/(Vbottom phase*ODbottom phase)
This formula uses 5 Variables
Variables Used
The yield of protein - The yield of protein is the amount of protein extracted by aqueous two phase extraction.
The Volume of top phase - (Measured in Cubic Meter) - The Volume of top phase is determined by the initial ratio of the two phases and the conditions of the extraction process.
The optical density of top phase - (Measured in Cubic Meter) - The optical density of top phase is the optical density of top phase extracts in aqueous two phase extraction.
The Volume of bottom phase - (Measured in Cubic Meter) - The Volume of bottom phase is the volume of liquid collected in the bottom phase after two phase extraction.
The optical density of bottom phase - (Measured in Cubic Meter) - The optical density of bottom phase the optical denisty of the bottom phase extracts in aqeous two phase extraction.
STEP 1: Convert Input(s) to Base Unit
The Volume of top phase: 25 Milliliter --> 2.5E-05 Cubic Meter (Check conversion here)
The optical density of top phase: 25 Milliliter --> 2.5E-05 Cubic Meter (Check conversion here)
The Volume of bottom phase: 20 Milliliter --> 2E-05 Cubic Meter (Check conversion here)
The optical density of bottom phase: 20 Milliliter --> 2E-05 Cubic Meter (Check conversion here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
Yyield of protein = (Vtop phase*O.Dtop phase)/(Vbottom phase*ODbottom phase) --> (2.5E-05*2.5E-05)/(2E-05*2E-05)
Evaluating ... ...
Yyield of protein = 1.5625
STEP 3: Convert Result to Output's Unit
1.5625 --> No Conversion Required
1.5625 <-- The yield of protein
(Calculation completed in 00.004 seconds)
You are here -
Home »

## Credits

Created by harykrishnan
SRM Institute of Science and Technology (SRMIST), Chennai
harykrishnan has created this Calculator and 10+ more calculators!
Verified by Soupayan banerjee
National University of Judicial Science (NUJS), Kolkata
Soupayan banerjee has verified this Calculator and 800+ more calculators!

## < 24 Microbiology Calculators

Narrow Heritability using Breeder's equation
Narrow Sense Heritability = var(Additive Genetic of (Aa) Allele,Additive Genetic of Allele (AA),Additive Genetic of (aa) Allele)/var(Phenotype of (aa) Allele,Phenotype of (AA) Allele,Phenotype of (Aa) Allele)
Broad Sense Heritability = var(Genotype of (Aa) Allele,Genotype of (aa) Allele,Genotype of (AA) Allele)/var(Phenotype of (aa) Allele,Phenotype of (AA) Allele,Phenotype of (Aa) Allele)
Protein Release Constant
The release constant = ln(The protein content maximum)/(The protein content maximum-The protein release fractional)/The sonication time
Yield of Protein
The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase)
Lineweaver Burk plot
The initial reaction rate = (The maximum reaction rate*The substrare concentration)/(Michaelis Constant+The substrare concentration)
Heat Generated during Microbial Growth
Metabolic heat evolved = (Substrate yield coefficient)/(Heat of combustion-Substrate yield coefficient*Heat of combustion of cell)
Rotational Angle of Alpha Helix
Rotation Angle per Residue = acos((1-(4*cos(((Dihedral angles around negative 65°+Dihedral Angles around negative 45°)/2)^2)))/3)
Temperature Coefficient of Resistance of RTD
Temperature Coefficient of Resistance = (Resistance of RTD at 100-Resistance of RTD at 0)/(Resistance of RTD at 0*100)
Hardy-Weinberg Equilibrium Equation for Predicted Frequency of Heterozygous (Aa) Type
Predicted Frequency of Heterozygous people = 1-(Predicted Frequency of Homozygous Dominant^2)-(Predicted Frequency of Homozygous Recessive^2)
Hardy Weinberg Equation for Predicted Frequency of Homozygous Dominant (AA) Type
Predicted Frequency of Homozygous Dominant = 1-(Predicted Frequency of Heterozygous people)-(Predicted Frequency of Homozygous Recessive)
Net Specific Replication Rate
Net specific replication rate = (1/Cell mass concentration)*(Change in mass concentration/Change in time)
Net Specific Growth Rate of Bacteria
Net specific growth rate = 1/Cell mass concentration*(Change in mass concentration/Change in time)
Fugacity Capacity of Chemical in Fish
Fugacity Capacity of Fish = (Density of Fish*Bioconcentration Factors)/Henry Law Constant
Fitness of Group i in Population
Fitness of Group i = Number of Group i Individuals in Next Generation/Number of Group i individuals Previous Generation
Protein release by cell disruption
The protein release fractional = The protein content maximum-The protein concentration at specific time
Wall tension of Vessel using Young-Laplace Equation
Hoop Stress = (Blood Pressure*Inner Radius of Cylinder)/Wall Thickness
Percentage Protein Recovery
The protein recovery = (The final concentration of protein/The intial concentration of protein)*100
Partition coefficeint of protein
The partition coefiicient = The optical density of top phase/The optical density of bottom phase
Bioconcentration Factor
Bioconcentration Factors = Concentration of Metal in Plant Tissue/Concentration of Metal in Soil
Octanol-Water Partition Coefficient
Octanol-Water Partition Coefficient = Concentration of Octanol/Concentration of Water
Net Specific Growth Rate Cell Death
Net specific growth rate = Gross specific growth rate-Rate of loss of cell mass
Solute Potential of Cell given Water and Pressure Potential
Solute Potential = Water Potential-Pressure Potential
Pressure Potential of Cell given Water and Solute Potential
Pressure Potential = Water Potential-Solute Potential
Approximate Water Potential of Cell
Water Potential = Solute Potential+Pressure Potential

## Yield of Protein Formula

The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase)
Yyield of protein = (Vtop phase*O.Dtop phase)/(Vbottom phase*ODbottom phase)

## What is Aqueous Two Phase Extraction ?

Aqueous two-phase extraction is a liquid-liquid separation technique that uses two immiscible aqueous solutions to partition and separate biomolecules or other substances based on their affinity for the different phases.

## What is Protein Yield ?

Protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases.

## How to Calculate Yield of Protein?

Yield of Protein calculator uses The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase) to calculate the The yield of protein, The Yield of Protein is the amount of protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases. The yield of protein is denoted by Yyield of protein symbol.

How to calculate Yield of Protein using this online calculator? To use this online calculator for Yield of Protein, enter The Volume of top phase (Vtop phase), The optical density of top phase (O.Dtop phase), The Volume of bottom phase (Vbottom phase) & The optical density of bottom phase (ODbottom phase) and hit the calculate button. Here is how the Yield of Protein calculation can be explained with given input values -> 1.25 = (2.5E-05*2.5E-05)/(2E-05*2E-05).

### FAQ

What is Yield of Protein?
The Yield of Protein is the amount of protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases and is represented as Yyield of protein = (Vtop phase*O.Dtop phase)/(Vbottom phase*ODbottom phase) or The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase). The Volume of top phase is determined by the initial ratio of the two phases and the conditions of the extraction process, The optical density of top phase is the optical density of top phase extracts in aqueous two phase extraction, The Volume of bottom phase is the volume of liquid collected in the bottom phase after two phase extraction & The optical density of bottom phase the optical denisty of the bottom phase extracts in aqeous two phase extraction.
How to calculate Yield of Protein?
The Yield of Protein is the amount of protein yield in aqueous two-phase extraction is the amount of target proteins successfully separated and recovered from a sample using the liquid-liquid extraction method with two immiscible aqueous phases is calculated using The yield of protein = (The Volume of top phase*The optical density of top phase)/(The Volume of bottom phase*The optical density of bottom phase). To calculate Yield of Protein, you need The Volume of top phase (Vtop phase), The optical density of top phase (O.Dtop phase), The Volume of bottom phase (Vbottom phase) & The optical density of bottom phase (ODbottom phase). With our tool, you need to enter the respective value for The Volume of top phase, The optical density of top phase, The Volume of bottom phase & The optical density of bottom phase and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
Let Others Know